<< Return to News

Continuous Development of Molecular Methods To Solve Your Aquaculture Customers Pain

MDG has made continuous advances in molecular methods for the aquaculture industry. Over the last year, we presented three posters that focus on how these methods provide better insight for your customers. Thanks for all of those who dropped by our poster at the World Aquaculture Society meetings in Jeju, Korea and also our poster at the American Society of Microbiology in New Orleans, USA.  If you weren’t there, here is a brief synopsis of our work.

Poster #1

Combining the use of Whatman FTA cards and diagnostic PCR assays to determine the pathogenic potential of shrimp water samples shipped overseas.  2014. S. Son,

newsletter_1

A. Lange, H. Behn, and M. King.  Abstract/Poster:  Aquaculture America 2014.  Seattle, WA.

This poster was our first development of a pathogen detection method that allowed us to analyze your customers aquaculture sample from anywhere in the world. Simply put one drop of aquaculture pond water on a FTA card let it dry, and mail it without losing

microbial diversities or paying for expensive international shipping charges.

From this card we are able to extract DNA from the bacteria in the pond water and identify the Vibrio toxins present in the ponds sampled.This method was semi-quantitative and allowed us to provide meaningful information to your customers but required extra work to provide numerical values vs presence/absence information.  However, it was the cornerstone of being able to receive samples globally.

If interested, this work is in print. Field Diagnostic Tools for Pathogenic Vibrios.    
M. King, S. Son, A. Lange, H. Behn, and J. Bradley.  2014.  Global Aquaculture Advocate. May/June.  40-42

Poster #2

Use of FTA cards for quantitative PCR detection of Vibrio harveyi and Vibrio campbellIi toxin genes and gene expression within shrimp pond water samples.  2015. Sona Son*, Amy M. Lange, and Michael R. King.  Abstract:  World Aquaculture Society Meeting.  Jeju, Korea.

The next step of adapting our pathogen method to quantitative PCR (qPCR) focused on poster 2reducing labor-intensive work and obtaining more precise information when determining the amount of pathogenic toxins in a shrimp pond. This advancement allowed us to rapidly and quantitatively detect toxin genes and infection rates on the globally shipped FTA card.

This work started at the DNA level, where we recognized that just because a toxin gene is present, it doesn’t mean the gene is turned on.  Meaning, the pathogen may not be the cause of disease in certain conditions. We began to show that poor management practices, such as not maintaining proper pH or excessive or inadequate salinity, actually induce pathogenic Vibrio to produce their toxins, and that the same bacteria in well-managed conditions did not produce those toxins. Vibrio seem to be friendly when conditions are ideal, but when conditions go beyond optimal, the toxins turn on. With this additional information and the use of the FTA card we are able to provide a better risk assessment for producers.

Poster #3

Use of FTA cards for diagnostic reverse transcriptase PCR to determine Vibrio harveyihemolysin toxin gene expression in shrimp pond samples. 2015. A. Lange, S. Son, M. King. Abstract. American Society of Microbiology General Meeting.  New Orleans, LA.

Our third poster, which was presented at the American Society of Microbiology conference in New Orleans, LA, begins to provide the application of all our previous efforts.  After showing that we could detect differences in toxin gene expression in the laboratory, we poster 3sought to study how stable RNA would be on a card shipped around the world.  We found that the RNA involved in toxin production was indeed stable and that we can study changing levels of gene expression in field samples.   This permits us to show the cause and effect relationship between the environmental factors, management practices, and the use or non-use of a bioaugmentation product in a pond and the changes in health and productivity we observe.

The information that we are able to obtain through the FTA card is essential in providing insight to improve your customers overall yield which is often negated by Vibrio in improperly managed systems.

 

These tools will begin to demonstrate what pathogens are present, and their ability to turn on their toxin genes without notice.  Showing where and how problems exist is the first step toward ounce of prevention vs a pound of cure. Molecular methods, such as those found in our three posters, allow us to prevent the problems of tomorrow vs. fighting the problems we face today. As the world starts to slip back into less water exchange strategies, these types of management programs become more important in finding ways to deal with shrimp pathogens.

 

If you’re interested in more information or sample testing we would be glad to hear from you. No matter where you are in the world.  

Search Insights
Recently Posted Insights

MDG Exhibits at the IWA World Water Congress & Exhibition 2024

Every year, we participate in trade shows and exhibitions around the globe, showcasing our innovative microbial solutions in the wastewater industry. This year, we will be attending the International Water Association (IWA) World Water Congress & Exhibition from...

How to Help Your Wastewater Customers Prepare for Winter

The changing of the seasons can have a pronounced effect on wastewater systems, particularly when the weather gets cold. Lower temperatures correspond to a decrease in microbial activity, which slows the treatment process. If you’re looking for a solution for your...

The Importance of Strain Purity Testing in Custom Fermentation

At MDG, we are Bacillus experts with over 20 years of experience growing pure cultures for our customers. We are continually honing our methods to find the most effective, efficient way to create quality microbial ingredients, and one contributing factor is purity...

A Guide to Wastewater Success: People, Products, and Programs

Working in the wastewater treatment industry comes with its challenges. Your customers may face problems like sludge buildup, odors, hydrogen sulfide (H2S) production, and fats, oils, and grease (FOG) accumulation. As their supplier, you need a solution you can count...

The Commercialization of Microbial Solutions

As a Bacillus manufacturer, we work to grow our partners’ pure single-strain Bacillus cultures and scale the process so that they receive exactly what they need. Our success in the commercialization of microbial solutions comes from our trusted process and our...

How to Handle Odors Within Septic Systems After Spring Thaw

Spring’s warmer weather and longer days are always welcome after a cold winter, but for septic tank owners and operators, this seasonal transition can bring a significant problem: odors. Now that spring thaw has occurred and the heat of summer is setting in, septic...

In-Field and In-House Expertise in Wastewater Treatment

When your wastewater customers are facing challenges, you want a partner with proven success and extensive expertise in wastewater treatment. At Microbial Discovery Group, we can be that partner. MDG stands as a leading expert in bioaugmentation, holding in-field and...

2024 EXPANSION: CONTINUED GROWTH AT MDG

Since the completion of our Oak Creek facility in the fall of 2022, we have steadily grown our capacities and capabilities as a trusted provider of Bacillus-based solutions. We are excited to announce that our growth has given us the opportunity to expand our...

Related News