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    [post_content] => Typically, one of the first questions a contract fermentation customer will ask us is how quickly we can get them to economical commercial production. From our years of experience, we commonly find that if the customer can get 2 Billion CFU/ml (2B) in a standard media such as Trypticase Soy Broth (TSB) or Nutrient Broth (NB), we tend to be able to get them to commercial production in 8-12 weeks.

However, if the customer is in the 107 or 108 CFU/ml range in this quick test, we know we have a good amount of work ahead of us and it may take a while. Mind you, we have brought many strains with lower than “2B” CFU/ml yield to a point of economic feasibility, but you can bet it increases the production timeline quite a bit. Now, we know the last thing a customer wants to hear is that production yields may delay or even prevent commercialization all together. In order to prevent this, we highly recommend adding a simple 2B TSB screen into your earlier processes.

Planning Ahead

Right now, many scientists are trying to find the perfect strain for their application. They will spend months if not years screening their Bacillus libraries seeking desired attributes such as anti-fungal activity, plant growth promotion properties, hydrocarbon degradation or enzyme production properties. Once they find this wonder strain, the temptation is to immediately run to field trials or begin the arduous patenting process, both of which can take years to complete.

While you may be excited to run with your new proprietary strain, we urge you to take a precautionary step by performing an additional assay or 2B TSB screen when choosing between your library strains. We have seen many cases with customer strain candidates, where a scientist had invested 5-7 years of lab and fieldwork, only to find out in the end, economical production was not going to happen with us or other contract manufacturers. At the end of the day, some strains just do not want to scale up to some of these higher concentrations.

By adding this important test within your library strain selection processes, you could find that you may be better off choosing the strain with maybe 90-95% of the antifungal producing attribute compared to your best strain. That is if it also performs well during the 2B fermentation quick screen.

2B TSB Screen

When implementing this test, we would suggest taking a simple laboratory media such as Trypticase Soy Broth or Nutrient Broth and screen your top strains for the ability to achieve 2 Billion CFU/ml of spores in 48-72 hrs of aerobic growth (200-250 rpm shaking in a baffled flask). If you can achieve that number, you are in luck. You will likely move very quickly to an organism yielding 12-15 Billion CFU/ml with a chance of 20-30 Billion CFU/ml in the commercial fermenter. This will translate to 4x1011 to 2x1012 CFU/gram of freeze or spray dried powder in 8-12 weeks.

Before You Go to the Field, Check the Fermentation Yield

In summary, we know the pain of having invested so much time into your favorite strain only to find out that production yield will delay or prevent commercialization.  Once you have gone deep into field trials or patenting, it is so hard to turn back. Therefore, our advice is to add this screen way back during the library development stage. Do yourself a favor, take the extra couple of weeks to test your strains for economic viability. You will win in the end if you test for “2B or not 2B”.

About the Author:

Michael R. King, Ph.D., is CEO/CSO and the founder of Microbial Discovery Group (MDG). Michael is passionate about leading and developing dynamic teams in the Franklin and Oak Creek, WI facilities who share a common vision to Feed, Clean and Save the World.

Dr. King received both his Bachelor of Animal Science and Masters of Food Science Microbiology degrees from Oklahoma State University, followed by a PhD in Food Science Microbiology from the University of Illinois. Prior to founding Microbial Discovery Group, Michael held research and leadership positions within Nalco Chemical Company, Semco Bioscience and Agtech Products. With 25+ years of experience in microbial biosciences, Michael is committed to the continued exploration and advancement of biological solutions that leave the planet in a better place for generations to come.
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News

2B or Not 2B? Success Criteria for Economical Bacillus Scale-Up

  • Mike King
  • January 20, 2021
Typically, one of the first questions a contract fermentation customer will ask us is how quickly we can get them to economical commercial production. From our years of experience, we commonly find that if the customer can get 2 Billion CFU/ml (2B) in a standard media such as Trypticase Soy Broth (TSB) or Nutrient Broth (NB), we tend to be able to get them to commercial production in 8-12 weeks. However, if the customer is in the 107 or 108
-READ MORE-

Under the Microscope, MDG’s Industrial & Institutional Sales Manager: Jenna Trusso

  • MDG
  • November 25, 2020
Jenna Trusso, Industrial & Institutional Sales Manager, is always ready to offer a helping hand. We recently sat down with the head of our Feed Clean Save program to learn more about how it has grown and what's planned for the future What is the MDG Feed Clean Save Committee? MDG’s vision is to Feed, Clean and Save the World. While our products do that each and every day, our employees also like to extend efforts locally through
-READ MORE-

New Lagoon Microbial Treatment

  • MDG
  • November 23, 2020
Franklin, WI - Microbial Discovery Group (MDG), a leading Bacillus fermentation company, announced the newest addition to their Biotifx® wastewater treatment line – 40B Water Soluble Packets. The Biotifx 40B Water Soluble Packet is a highly concentrated, microbial treatment for lagoons and other large wastewater systems. The powder is pre-measured and the packaging dissolves in water, making it easy for operators to apply. Effective
-READ MORE-

How to Increase Your Sales

  • MDG
  • November 17, 2020
Could you benefit from higher income per sales hour? Lower hours spent per close? Higher close rates? This is what’s possible when you distribute quality, science-backed bioaugmentation solutions that are paired with an easy-to-understand, product education program for your sales team. Premium Solutions + Effective Training = Success At MDG, we’ve taken the time to work with the scientific community to not only understand what’s
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Cleaning with Biosurfactants

  • MDG
  • November 6, 2020
In your search for environmentally friendly cleaning ingredients, you’ve likely come across the term ‘biosurfactants’. Do you know what they are – and what they do in bio-enzymatic cleaners? Read on to become armed with the knowledge you need to make informed formulation decisions. What is a Biosurfactant? A biosurfactant is a biologically derived substance that helps reduce the surface tension of a liquid in which it’s
-READ MORE-

Resolving Toxic Upsets: Quats in Wastewater

  • MDG
  • October 15, 2020
Earlier in this article series, we touched on how bioaugmentation treatments for wastewater have been around for 50+ years to help resolve toxic upsets. We also explored how Bacillus, a certain genus of bacteria that can be used in microbial treatments, are resilient to oxidizers. Today, we’ll examine how Bacillus are resistant to another powerful but non-oxidizing anti-microbial: Quats. What are Quats? Quaternary ammonium compounds
-READ MORE-

Winter Wastewater Treatment: Don’t Leave Your Customers Out in the Cold

  • MDG
  • September 24, 2020
When distributors participate in our Biotifx® program, they become armed with the knowledge and confidence they need to support their customers with superior microbial wastewater treatments – even during the winter months. The program includes onsite training, straightforward product application guides and tools, even an on-site technical service rep if/whenever needed. And cool products too. Help Improve Cold Weather Performance A few
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Optimizing WAS Holding Systems

  • MDG
  • September 18, 2020
Before we walk through how to help your customers optimize their WAS holding systems, it's important to understand how they work. How does a WAS Holding System work? Waste Activated Sludge (WAS) holding systems are a common part of operations in wastewater treatment plants (WWTPs). A WAS holding system, typically in the form of a sludge tank or lagoon, holds sludge after it has been wasted or removed from a clarifier. WAS systems may have
-READ MORE-

What’s Inside a Bio-Enzymatic Cleaner?

  • MDG
  • September 16, 2020
With environmentally friendly cleaners continuing to trend, more and more chemical manufacturers want to know – what’s inside a bio-enzymatic cleaner? In this article, we’ll walk through some of the common building blocks within formulations. Not all ingredients are essential for every product as it depends upon the application, but by understanding each element, you’ll know what you may want to request when discussing products
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Are You Offering a Bacillus RV Septic Treatment?

  • MDG
With social distancing still the norm, outdoor activities like RV camping are providing much needed freedom from indoor seclusion. While campfires, cooking outdoors, hiking and going wherever the road takes you are everyone’s favorite – sleeping in an RV with a smelly toilet isn’t! As a cleaning solutions manufacturer and/or distributor, you may know that there are three main types of RV septic tank treatments on the market:
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